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Original Research Article | OPEN ACCESS

Over-expression of miR-124 impairs proliferation, invasion and epithelial-mesenchymal transition in pituitary prolactinoma by targeting PHD finger protein 19

Zongxi Li1, Lingxuan Ren2, Shoujie Wang3

1Department of Neurosurgery, Clinical Medical College and The First Affiliated Hospital of Chengdu Medical College, Chengdu City, Sichuan Province 610500; 2Department of Child Healthcare, Cheng Du Xin Du Maternal & Child Health Care Hospital, Chengdu City, Sichuan Province 610500; 3Department of Neurosurgery, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an City, Shaanxi Province 710038, China.

For correspondence:-  Shoujie Wang   Email: sjwang2366@163.com   Tel:+862984717846

Accepted: 8 April 2021        Published: 30 April 2021

Citation: Li Z, Ren L, Wang S. Over-expression of miR-124 impairs proliferation, invasion and epithelial-mesenchymal transition in pituitary prolactinoma by targeting PHD finger protein 19. Trop J Pharm Res 2021; 20(4):721-726 doi: 10.4314/tjpr.v20i4.9

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the effect of miR-124 in pituitary prolactinoma.
Methods: The viability and proliferation of prolactinoma cells were investigated using Cell Counting Kit-8 and 5-ethynyl-2′-deoxyuridine staining assays. Cell migration and invasion were investigated using the transwell assay. The epithelial-mesenchymal transition was investigated using western blotting. The target gene of miR-124 was verified by the luciferase activity assay.
Results: The viability and proliferation of prolactinoma cells were repressed by miR-124 over-expression (p < 0.01). Forced miR-124 expression suppressed prolactinoma cell migration and invasion (p < 0.01). E-cadherin expression was enhanced, while N-cadherin and vimentin were reduced, by miR-124 over-expression (p < 0.01). PHF19 (plant homeodomain-like finger protein 19) contains an miR-124 binding site, and PHF19 over-expression enhanced cell proliferation, promoted cell migration and invasion, reduced E-cadherin expression and enhanced N-cadherin and vimentin expression in prolactinoma cells. Additionally, miR-124 mimic-induced suppression of prolactinoma cell growth and metastasis was attenuated by forced PHF19 expression.
Conclusion: MiR-124 retards prolactinoma cell growth and metastasis by reducing PHF19, providing a promising therapeutic target for prolactinoma.

Keywords: miR-124, PHF19, Pituitary prolactinoma, Cell proliferation, Cell invasion, Cell migration

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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